91麻豆精品一二三区在线,亚洲日韩中文字幕无码一区,久久精品成人无码观看56,国产精品久久久久久久久免费高清

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CCL-171MRC-5 人胚肺細(xì)胞

MRC-5 人胚肺細(xì)胞

簡要描述:CCL-171 MRC-5 人胚肺細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CCL-171
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:2152

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-171 MRC-5 人胚肺細(xì)胞

ATCC® Number:  CCL-171™

Designations:  MRC-5

Depositors:   JP Jacobs

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

CCL-171 MRC-5 人胚肺細(xì)胞

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation:  Isolation date: September, 1966

Applications: efficacy testing [92587]

testing [92346] [92389]

transfection host (Roche FuGENE® Transfection Reagents)

viruscide testing [92579] [92582]

Virus Susceptibility: poliovirus 1; herpes simplex; vesicular stomatitis (Indiana)

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11,14

D16S539: 9,11

D5S818: 11,12

D7S820: 10,11

THO1: 8

TPOX: 8

vWA: 15

Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal.

Isoenzymes:  G6PD, B

Age:  14 weeks gestation

Gender:  male

Ethnicity:  Caucasian


Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

CCL-171



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
中文字幕精品无码一区二区三区| 色狠狠av| 欧美6一10sex性hd| 亚洲乱亚洲乱妇无码| 不卡av一区二区中文字幕| 国产色A∨在线看免费| 日韩av电影网址| 麻豆精品一区二区| 人人讲| 看av| 久久精品成人无码观看| 亚洲精品中文字幕无码蜜桃| a级毛片无码免费真人久久| 欧美亚洲国产精品久久蜜芽直播 | 久久蜜桃| 色噜噜狠狠一区二区三区| 日皮视频| 孕妇孕交| 禄劝| 亚洲爆乳www无码专区| 宁陵县| 97视频精品全国免费观看| 化德县| 久久综合久久自在自线精品自| 亚洲综合小说另类图片动图| 蜜芽亚洲av无码精品国产午夜| 辽阳市| 亚洲国产精品无码av| 无码熟妇人妻AV在线影片最多| 国产免费久久精品国产传媒| 久久久亚洲欧洲日产国码是av| 国产av一区二区精品久久| 天天天天噜在线视频| 国产午夜精品一二区理论影院| 欧美高难度牲交视频| 亚洲欧洲精品成人久久曰 | 久久精品国产亚洲AV麻豆王友容| 亚洲欧美精品一中文字幕| 啦啦啦www在线观看免费视频| 乱公和我做爽死我视频| 激情综合色五月丁香六月欧美 |