91麻豆精品一二三区在线,亚洲日韩中文字幕无码一区,久久精品成人无码观看56,国产精品久久久久久久久免费高清

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>HTB-103KATO III細(xì)胞, 人胃癌細(xì)胞

KATO III細(xì)胞, 人胃癌細(xì)胞

簡(jiǎn)要描述:KATO III細(xì)胞, 人胃癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-103
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:1726

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

KATO III細(xì)胞, 人胃癌細(xì)胞

OrganismHomo sapiens, human
Tissue

stomach:derived from metastatic pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac

Product Formatfrozen
Morphologyspherical
Culture Propertiesmixed, adherent and suspension
Biosafety Level1
Diseasegastric carcinoma
Age55 years adult
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
KaryotypeThe stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected.
Clinical Data

55 years adult

Asian

male

TumorigenicYes
Effects

Yes, in cheek pouches of anti thymocyte serum treated hamsters

No, in nude mice

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
KATO III細(xì)胞, 人胃癌細(xì)胞
Subculturing

Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove culture medium with floating cells to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 2.. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 4. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximay 125 xg for 5 to10 minutes. 5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Place culture vessels in incubator at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: 5% CO2 in air recommended

Temperature: 37°C          






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
岛国岛国免费V片在线观看| 亚洲国产精品尤物YW在线观看| 亚洲一区综合| 午夜福利国产在线观看1| 四虎永久地址www成人| 欧美熟妇xxxx×欧美妇色| 国产精品久久久久久亚洲毛片| 久久久中文| 久久人人爽人人爽人人片av高请| 久久sese| 在线看片免费人成视频播| 亚洲国产精品国自产拍AV| 一本色道久久东京热| 亚洲人成网站日本片| 阳高县| 曲沃县| 观看国产色欲色欲色欲www | 国产成人精品2021| 亚洲av综合av一区二区三区| 在线亚洲高清揄拍自拍一品区| 国产曰的好深好爽免费视频| 亚欧洲精品在线视频免费观看| 句容市| 亚洲一区日韩高清中文字幕亚洲 | 蜜桃成熟期| 精品欧美一区二区三区精品久久| 老妇裸体性激交老太视频| 黑人亨特玩人妻杨晓蓉| 日韩a区| 色站综合| av中文网| 2020最新久久久视精品爱| 通榆县| 中文字幕乱偷无码动漫av| 国产成人啪精品午夜网站| 欧美亚洲另类丝袜综合网| 中文字幕人成乱码在线观看| 欧洲美熟女乱av在| 国产天美传媒性色AV出轨| 国产人成视频在线观看| 国产精品无码2021在线观看|