91麻豆精品一二三区在线,亚洲日韩中文字幕无码一区,久久精品成人无码观看56,国产精品久久久久久久久免费高清

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>Panc 03.27 人胰腺癌細(xì)胞

Panc 03.27 人胰腺癌細(xì)胞

簡要描述:CRL-2549 Panc 03.27
人胰腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
Panc 03.27 人胰腺癌細(xì)胞

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-26
  • 訪  問  量:1920

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

ATCC® Number: CRL-2549™ Price: $417.00

Designations: Panc 03.27

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

Tumorigenic: Yes

Oncogene: K-ras + (wild-type)

Antigen Expression: MHC class I +; MHC class II - [50655]

Blood type A; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12

D5S818: 12,13

D7S820: 8,13

THO1: 6

TPOX: 8,9

vWA: 16,17

Age: 65 years

Gender: female

Ethnicity: White

Comments: Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 37.5 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602


CRL-2549





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品毛片频| 久久久大片| 欧乱色国产精品兔费视频 | 国产乱子影视频上线免费观看| 中文字幕乱码无码人妻系列蜜桃| 我与么公激情性完整视频在线观看| 国产精品免费观看久久| 狠狠躁夜夜躁| 日韩AV高清无码| 国产成人久久精品77777综合 | 久久精见国产亚洲AV高清热| А√天堂资源中文在线地址BT| 国产精品亚韩精品无码a在线 | 日韩精品无码一区二区三区不卡| 性色AV免费网站| 宿迁市| 欧美大屁股熟妇性hd| 黑丝一区二区| 国产av剧情md精品麻豆| 狠狠躁夜夜躁无码中文字幕 | 97SE狠狠狠狠狼鲁亚洲综合色| 亚洲色爱图小说专区| 亚洲国产AV无码精品| 欧美大荫蒂毛茸茸视频| 国产亚洲美女精品久久久久| 在线精自偷自拍无码| 狠狠色综合激情丁香五月| 激情综合色五月丁香六月欧美 | 亚洲成AV人片天堂网久久| 九色国产| 日韩福利| 国产精品乱码一区二区| 狠狠躁日日躁夜夜躁| 精品国产一二三区| 亚洲欧美偷拍另类a∨色屁股| 久久男女| 国产精品欧美一区二区三区不卡| 免费99精品国产自在在线| 狠狠婷婷综合久久久久久| 人人干av| 亚洲乱轮|