91麻豆精品一二三区在线,亚洲日韩中文字幕无码一区,久久精品成人无码观看56,国产精品久久久久久久久免费高清

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠腫瘤細(xì)胞、癌細(xì)胞>TIB-67J774A.1 小鼠腹水單核細(xì)胞瘤

J774A.1 小鼠腹水單核細(xì)胞瘤

簡(jiǎn)要描述:TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):TIB-67
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2165

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件

TIB-67 J774A.1 小鼠腹水單核細(xì)胞瘤

ATCC® Number:TIB-67™  Price:$256.00
Designations:J774A.1

Depositors:P Ralph

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:macrophage
J774A.1 小鼠腹水單核細(xì)胞瘤


Source:Tissue: ascites
Strain: BALB/cN
Disease: reticulum cell sarcoma
Cell Type: monocyte/macrophage macrophage;


Cellular Products:interleukin 1 beta

lysozyme [1080]



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
TIB-67

Isolation:La Jolla California, United States
Isolation date: 1968


Applications:Biological response [92560]

transfection host (Roche FuGENE® Transfection Reagents)



Receptors:complement (C3), expressed [1135]

Fc receptor, IgG, high affinity I (Fcgr1), expressed [13710]



Age:adult

Gender:female

Comments:J774A.1 cells are active in antibody dependent phagocytosis [Pubmed: 1101071]. Their growth is inhibited by dextran sulfate, PPD and LPS [Pubmed: 318922]. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta (Il1b) is synthesized continuously by this line.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol: Subcultures are prepared by scraping.

For a 75 cm2 flask, remove all but 10 ml of the culture medium. (adjust volume accordingly for different culture vessels) Dislodge cells from the flask substrate with a cell scraper, aspirate and dispense into new flasks.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: Replace or add medium two or three times weekly


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:17 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

purified RNA:ATCC TIB-67R



References:1080: Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890

1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

1136: Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

13710: Sears DW, et al. Molecular cloning and expression of the mouse high affinity Fc receptor for IgG1. J. Immunol. 144: 371-378, 1990. PubMed: 2136886

22827: Ralph P, et al. Reticulum cell sarcoma: an effector cell in antibody-dependent cell- mediated immunity. J. Immunol. 114: 898-905, 1975. PubMed: 1089721

22896: Ralph P, Nakoinz I. Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line. Nature 257: 393-394, 1975. PubMed: 1101071

32697: Knowlton KU, et al. A mutation in the puff region of VP2 attenuates the myocarditic phenotype of an infectious cDNA of the woodruff variant of coxsackievirus B3. J. Virol. 70: 7811-7818, 1996. PubMed: 8892902

32883: Schissel SL, et al. Zn2+-stimulated sphingomyelinase is secreted by many cell types and is a product of the acid sphingomyelinase gene. J. Biol. Chem. 271: 18431-18436, 1996. PubMed: 8702487

92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
天天射日日射| 人妻在线无码一区二区三区| 久久8| 日视频| 色呦呦国产| 日韩首页| 欧美野外猛男的大粗鳮台湾同胞| 亚洲欧美a| 免费av在线| 少妇性饥渴无码A区免费| 啪啪AV| 亚洲成AⅤ人片精品久久久久久| 国产无AV码在线观看| 亚洲乱码一卡二卡卡3卡4卡| 欧美激情一区二区三区成人| 亚洲性色AV私人影院无码| 欧美大片va欧美在线播放| 久久人妻无码中文字幕| 97亚洲熟妇自偷自拍另类图片| 97无码视频在线看视频| 波多野结衣AV无码久久一区| 亚洲日韩一页精品发布| 国产成人免费av片在线观看| 国产成人AV一区二区三区无码| 色诱久久av| 欧美日韩精品一区二区三区不卡| 亚洲人成无码网站| 亚洲免费人成视频观看| 亚洲自偷自拍另类12p| 亚洲国产精品久久久久久久| 国产激情久久久久影院老熟女免费| 久久综合给合综合久久| 999国产精品999久久久久久| 最近日本中文字幕免费完整| 日韩精品一区二区午夜成人版| 你懂的国产在线精品| 色窝窝无码一区二区三区色欲| 久久精品中文骚妇内射| 老熟女另类xxx精品视频| 国内精品偷拍| 毛片一|