91麻豆精品一二三区在线,亚洲日韩中文字幕无码一区,久久精品成人无码观看56,国产精品久久久久久久久免费高清

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株

CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株

簡要描述:CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株,
CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,
細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2453

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和i優(yōu)培養(yǎng)條件


CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株

ATCC® Number: CCL-1.3™    Price: $329.00
Designations: L-M(TK-) [LM(tk-), LMTK-]

Depositors: TR Chen

Biosafety Level:1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast


Source:Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/An


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Tumorigenic:Yes

Antigen Expression:H-2k

Cytogenetic Analysis:modal number = 45; range = 42 to 48.

The stemline chromosome number is hyperdiploid with the 2S component occurring at 3.8%. Twenty-one markers were common to nearly all S metaphases studied. Of these, 13 were two-armed chromosomes, and eight, acrocentric. Four markers were isochromosomes by Robertsonian translocations from chromosomes 4, 12, 14 and 15. Normal X, 12, 14, 15 and 16 were absent. The X chromosome was present as an arm of Rb(X.3). (See Cancer Genet. Cytogenet. 24: 95-107, 1987 for a detailed karyotype description.)



Age: 100 days

Gender: male

Comments:The cells are 5-bromo-2'-deoxyuridine resistant (BUDR, BrdUrd) and thymidine kinase (TK) deficient.

HAT sensitive.

Tested and found negative for ectromelia virus (mousepox).



Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing: Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.


  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week


Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-1



References:868: Kit S, et al. Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine. Exp. Cell Res. 31: 297-312, 1963. PubMed: 14056989

21410: Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673

25904: Hsu TC, Somers CE. Properties of L cells resistant to 5-bromodeoxyuridine. Exp. Cell Res. 26: 404-410, 1962. PubMed: 14449548

25907: Boone C, et al. Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids (thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes). Proc. Natl. Acad. Sci. USA 69: 510-514, 1972. PubMed: 4110482

25910: Peterson JL, McBride OW. Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. USA 77: 1583-1587, 1980. PubMed: 6929511




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产97成人亚洲综合在线观看| 久久国产精品一区| 性xxxx欧美老妇506070| 国产精品高清一区二区三区| 日本做受高潮好舒服视频| 国产精品青青草原免费无码| 亚洲制服无码一区二区三区| 99精产国品一二三产区区别网站| 平泉县| 孽欲| 人妻少妇被粗大爽ⅹxoo| 国产精品人妻一码二码| 亚洲综合一区| 日本在线天堂| 亚洲一区国产| 麻豆一二三区av精品传媒软件| 懂色av蜜臀av粉嫩av| 欧美午夜特黄aaaaaa片| 91免费看国产| 97人妻精品一区二区三区视频| 欧美日韩精品| 成人午夜精品| 狼人色综合| 日本久久久久久久| 免费麻豆| 国产精品一区二区三区在线| 午夜精品久久久久| www.黄视频| 另类另类另类另类性| 国产精品久久久久久亚洲影视| 激情内射亚洲一区二区三区| 亚洲av乱码专区国产乱码| AV免费播放一区二区三区| 国产v亚洲v欧美v专区| 99久久婷婷国产一区二区| 一本久道综合在线无码88| 人人澡人人妻人人爽人人蜜桃| 伊人激情av一区二区三区| 国产亚洲一本大道中文在线| 亚洲伊人成无码综合影院| 久久久久国产精品熟女影院|